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In this embodiment, the TetR and revTetR encoding genes are operatively associated with different genetic regulatory elements providing independent expression of each type of repressor protein?
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"Difference apparently go Between Ointment and Cream" DifferenceBetweennet? In other embodiments, the isolated nucleic acids comprise a nucleotide sequence encoding modified revTetR proteins that exhibit the reverse phenotype in prokaryotes only at particular temperatures, eg, exhibit the reverse phenotype only at 28° C.

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